SFRBM's 17th ANNUAL MEETING PROGRAM,
a joint meeting with SFRRI
Program subject to change (as of November 2, 2010)
WEDNESDAY, NOVEMBER 17, 2010
8:30 am – 3:40 pm
PRE-MEETING WORKSHOP I
New Approaches for Examining Nitrative and Oxidative Stress in Biology
Chairs: Joe S. Beckman, Ph.D., Linus Pauling Institute and Balaraman Kalyanaraman, Ph.D., Medical College of Wisconsin
Major experimental advances made in the past several years can
fundamentally transform our ability to critically examine the biological
role of nitrative and oxidative stress in biology and medicine. New
fluorescent probes can detect superoxide and peroxynitrite production in
vivo, but only if carefully applied. Nitration, hydroxylation and
dimerization of protein-bound tyrosines have become a widely used marker
of oxidative stress. New approaches for understanding the specificity
for certain tyrosine sites to be susceptible to nitration will allow the
biological implications of tyrosine modifications to be characterized.
Importantly, the specific functional consequences can be determined by
directly incorporating nitrotyrosine into recombinant proteins by
unnatural amino acid mutagenesis. Mitochondria are key targets of
oxidative stress. New technologies of monitoring mitochondrial function
are transforming our understanding of their dysfunction in vivo. These
new approaches can provide mechanistic insights into how oxidants
specifically damage cells to produce pathological changes that underlie
so many disease processes.
8:30 am – 9:00 am
New Advances to Explore Nitrative and Oxidative Stress in vivo
Joe Beckman, Ph.D., Linus Pauling Institute
9:00 am – 9:30 am
Measuring Superoxide and Peroxynitrite in Cells: The “Do or Dye” Approach
Balaraman Kalyanaraman, Ph.D., Medical College of Wisconsin
9:30 am – 10:00 am
How to Measure Protein Oxidation and Nitration
Michael Davies, Ph.D., Heart Research Institute
10:00 am – 10:30 am
Coffee Break
10:30 am – 11:00 am
In vivo Measurements of Protein and DNA Radicals by Immuno-spin Trapping
Ronald Mason, Ph.D., NIEHS/NIH
11:00 am – 11:30 am
Assessing the Biological Impact of Increased Mitochondrial Oxidant Generation
Lee Ann MacMillan-Crow, Ph.D., University of Arkansas
11:30 am – 12:00 pm
Activation of a HSP90 Toxic Gain-in-function by Tyrosine Nitration: A Molecular Mechanism for Peroxynitrite Toxicity
Alvaro Estevez, Ph.D., Oregon State University
12:00 pm – 1:30 pm
Lunch
1:30 pm – 2:10 pm
The Application of Affinity Reagents to Detect Protein Post-translational Modifications
Aimee Landar, Ph.D., University of Alabama at Birmingham
2:10 pm – 2:50 pm
Relating Insult to Injury: How to Measure Oxidative Protein Modifications and Bioenergetics in Cardiovascular Cells
Bradford Hill, Ph.D., University of Louisville
2:50 pm – 3:20 pm
Using Redox Proteins as Redox Sensors of Cellular Oxidative Stress
Mark Hampton, Ph.D., University of Otago, New Zealand
3:20 pm – 3:40 pm
Closing Remarks: Where We Will Go Wrong? Critical Thinking About How These Techniques Could Be Misapplied.
ANNUAL MEETING BEGINS
5:00 pm – 6:45 pm
Celebrating 25 Years of Free Radical Biology and Medicine
Chair: Kelvin J. A. Davies, Ph.D., D.Sc., University of Southern California
It hardly seems possible that our society journal, Free Radical Biology
& Medicine was first published in 1985, but it’s true and this year
we will be celebrating its 25th anniversary! To kick-off the
festivities, we will have a special opening plenary session at the
meeting, featuring members of our FRBM’s Distinguished Editorial Board,
and former Associate Editors. Join Nobel Prize winners, National
Academy members, and National Medal of Science recipients, as we
celebrate the 25 year impact of our society journal on the development
of the field.
5:00 – 5:15 pm
SFRBM & SFRRI President’s Welcome
Victor Darley-Usmar, Ph.D., University of Alabama at Birmingham and Angelo Azzi, Tufts University
FRBM Introduction
Kelvin J. A. Davies, Ph.D., D.Sc., University of Southern California
5:15 pm – 5:45 pm
Flavonoids in 2010: From Antioxidant Capacity to Cardiovascular Functions
Balz Frei, Ph.D., Linus Pauling Institute
5:45 pm – 6:15 pm
Free Radicals in Biology and Medicine: Good, Unexpected, and Uninvited Friends
Etsuo Niki, Ph.D., National Institute of Advanced Industrial Science & Technology, Japan
6:15 pm – 6:45 pm
A Diet for Health and Longevity
Bruce N. Ames, Ph.D., UC Berkeley
7:00 pm – 9:00 pm
Welcome Reception
9:00 pm – 12:00 am
Hospitality
THURSDAY, NOVEMBER 18, 2010
SUNRISE FREE RADICAL SCHOOL
Chairs: Alicia Kowaltowski, Ph.D., University of São Paulo, Brazil and Aimee Landar, Ph.D.,University of Alabama at Birmingham
8:00 am – 8:30 am
Introduction to ROS in the Cardiovascular System
John Keaney, MD, UMASS Medical School
8:30 am – 9:00 am
Oxidative Stress and Cardiac Disease
Louis Dell‘Italia, MD, University of Alabama at Birmingham
PLENARY SESSION
Redox Signaling in the Pathogenesis of Cardiovascular Disease
Chairs: Philip Eaton, Ph.D., King’s College London, UK and Ajay Shah, MD, FRCP, FmedSci, King’s College London, UK
There is a growing recognition of the crucial role of redox signaling
in the pathogenesis of cardiovascular disease. It is important to define
precisely how changes in cellular oxidants and antioxidants contribute
to the development of hypertension, ischemic heart disease and the
growing burden of heart failure. This session will provide an update of
progress in this area from leaders in the field who study redox
alterations in cardiovascular dysfunction using powerful molecular
techniques together with highly relevant (often in vivo) disease models.
9:30 am – 10:00 am
Role of NOS in Cardiac Oxidative Stress and Pathologic Remodeling
David Kass, MD, The Johns Hopkins Medical Institutions
10:00 am – 10:30 am
Nox Enzymes and the Development of Heart Failure
Ajay Shah, MD, FRCP, FmedSci, King’s College London, UK
10:30 am – 11:00 am
Break
11:00 am – 11:30 am
Regulation of Thioredoxin-1 in Endothelial Cells
Judith Haendeler, Ph.D., University of Duesseldorf, Germany
11:30 am – 12:00 pm
Redox Regulation of Class II HDACs and Cardiac Hypertrophy
Junichi Sadoshima, MD, Ph.D., University of Medicine and Dentistry at New Jersey
12:00 pm - 12:30 pm
SFRBM Member Meeting
12:30 pm – 2:30 pm
Lunch attendees on own or attend a professional development session
12:45 pm – 1:45 pm
Professional Development Session: Grantsmanship Workshop
Edward Postlethwait, Ph.D., University of Alabama at Birmingham
This very successful session returns for 2010 with a renewed look at
the rapidly-changing subject of grant submission. In addition to
covering classical topics such as basic grantsmanship, the session will
also cover the recent substantial changes to the NIH grant writing and
scoring process, and will also provide attendees with a list of
resources and funding agencies. The workshop is aimed at investigators
at all levels.
12:45 pm – 1:45 pm
Professional Development Session: Intellectual Property…Turning Your Molecule Into a Drug
Bruce Freeman, Ph.D., University of Pittsburgh
This session will give attendees an introduction to the field of
patents, intellectual property protection, and commercialization of
ideas in the life sciences. The session is aimed at anyone interested in
taking their ideas in the lab’ into the realm of pharmaceutical
development, and will include discussions on how to time such
developments, what resources are available, attracting venture capital,
protecting your discovery, and how to keep your academic/commercial
enterprises separate.
2:30 pm – 4:30 pm
Oral presentations from submitted abstracts
(4 concurrent sessions)
4:30 pm – 6:30 pm
Formal Poster Presentations
6:30 pm – 9:30 pm
Opening Doors Event: Celebrating Cultural Diversity in Science
The “Opening Doors Event” provides an opportunity for education,
dialogue and networking among scientists at all stages of their careers.
This year’s Opening Doors Event will be in the form of a workshop, and
will focus on cultural diversity in the workplace. The components of
this workshop will include 1) recognizing our dissimilarities, 2)
celebrating our cultural differences, and 3) benefiting from our
diversities.
Organized by Aimee Landar, Ph.D, University of Alabama at Birmingham,
Xiaolei (Dawn) Jin, Health Canada and Sally Nelson, Ph.D., SomaLogic,
Inc., and the Women in Science Committee (WIS)
6:30 pm – 8:00 pm
FRBM Editorial Board Reception
9:00 pm – 12:00 am
Hospitality
FRIDAY, NOVEMBER 19, 2010
SUNRISE FREE RADICAL SCHOOL
Chairs: Alicia Kowaltowski, Ph.D., University of São Paulo, Brazil and Aimee Landar, Ph.D.,University of Alabama at Birmingham
8:00 am – 8:30 am
The Endoplasmic Reticulum: An Emerging Player in Redox Pathophysiology
Francisco Laurindo, MD, University of São Paulo
8:30 am – 9:00 am
From NO to Nitrite and Nitrate...and Back Again: Roles in Mammalian Biology
Rakesh Patel, Ph.D., University of Alabama at Birmingham
PLENARY SESSION
Role of Mitochondria and Reactive Species in Stem Cell Biology
Chairs: Bradford G. Hill, Ph.D., University of Louisville, and Toren Finkel, MD, Ph.D., National Heart Lung and Blood, NIH
The use of stem cells for treating disease is now entering the realm of
possibility, thanks to a better understanding of their biology. Recent
work has shown that the proliferation, homing, and differentiation of
stem cells rely in part on changes in metabolism and on reactive species
generated during the natural course of physiology or during the disease
process. The purpose of this plenary session will be to highlight
important contributions to this field and to initiate interest in the
role of reactive species and mitochondria in stem cell biology.
9:30 am – 10:00 am
Mitochondrial and Redox Regulation of Stem Cell Biology
Toren Finkel, MD, Ph.D., National Heart Lung and Blood, NIH
10:00 am – 10:30 am
Nitric Oxide-Cyclic GMP Signaling in Stem Cell Differentiation
Kalpana Mujoo, Ph.D., University of Texas Health Sciences Center at Houston
10:30 am – 11:00 am
Break
11:00 am – 11:30 am
Pluripotent Stem Cells and Mitochondrial Remodeling
Timothy Nelson, M.D., Ph.D., Mayo Clinic
11:30 am – 12:00 pm
Maintaining Stem Cell Fate During Drosophila Hematopoiesis
Utpal Banerjee, Ph.D., UCLA
12:00 pm – 12:30 pm
SFRBM Lifetime Achievement Lecture:
One-Electron and Two-Electron Signaling in Redox Biology
Garry Buettner, Ph.D., The University of Iowa
12:30 pm – 2:30 pm
Lunch attendees on own or attend a professional development session
12:45 pm – 1:45 pm
Professional Development Session: Getting Your Point Across – The Basics of a 15 minute Conference Lecture
Victor Darley-Usmar, Ph.D., University of Alabama at Birmingham
This
session starts with the preparation of abstracts and develops the
principles of presentations using power point as applied to research
presentations. The workshop is aimed at students, post-docs and
junior investigators.
12:45 pm – 1:45 pm
Professional Development Session: Publications…From Bench to Bookshelf
Balaraman Kalyanaraman, Ph.D., Medical College of Wisconsin and Anthony Newman, Elsevier, Inc.
This session is designed for investigators at all levels, and presents
an inside-out look at the world of scientific publishing, from the
perspective of both the publisher and an associate editor of FRBM. In
addition to covering broad topics such as manuscript preparation and how
to deal with reviewer’s comments, the workshop will also touch on
novel developments in publishing such as on-line technologies (Scopus,
Pubget, SML).
2:30 pm – 4:30 pm
Oral presentations from submitted abstracts
(4 concurrent sessions)
4:30 pm – 6:30 pm
Formal Poster Presentations
9:00 pm – 12:00 am
Hospitality
SATURDAY, NOVEMBER 20, 2010
SUNRISE FREE RADICAL SCHOOL
Chairs: Alicia Kowaltowski, Ph.D., University of São Paulo, Brazil and Aimee Landar, Ph.D.,University of Alabama at Birmingham
8:00 am – 8:30 am
Reactivity and Biological Functions of Oxidized Lipids
Matthew Picklo, Ph.D., USDA - ARS
8:30 am – 9:00 am
Properties of Protein Targets of Reactive (Lipid) Species
Dennis Petersen, Ph.D., University of Colorado
PLENARY SESSION
Biochemistry and Pathology of Reactive Lipid Species
Chairs: Henry Forman, Ph.D., University of California at Merced, and Corinne Spickett, University of Strathclyde, UK
The involvement of reactive, electrophilic lipid species in physiology
and pathology is becoming widely accepted, and recent advances
in mass spectrometry and proteomics have facilitated understanding
of the mechanisms involved. The ability to modulate pathways of cell
signaling and gene expression is emerging as critical. This session
will present the latest findings on how reactive lipid species influence
signaling processes, and will describe the technologies that have
underpinned these advances.
9:30 am – 10:00 am
Systems Approaches to Establishing the Relationship Between Protein Modification and Cellular Responses by Lipid Electrophiles
Larry Marnett, Ph.D., Vanderbilt University
10:00 am – 10:30 am
Protein Bound HNE as a Ligand of LOX 1
Koji Uchida, Ph.D., Nagoya University, Japan
10:30 am – 11:00 am
Break
11:00 am – 11:30 am
Inflammation Related Gene Expression by Lipid Oxidation Derived Products in the Progression of Atherosclerosis
Giuseppe Poli, MD, Ph.D., University of Turin, Italy
11:30 am – 12:00 pm
Activation of Stress Signaling Pathways by Oxidized and Nitrated Lipids
Anna-Liisa Levonen, MD, Ph.D., University of Eastern Finland, Finland
12:00 pm – 12:30 pm
Trevor Slater Lecture
Vitamin E: From Chemistry to Biology. A review and Perspective
Etsuo Niki, Ph.D., National Institute of Advanced Industrial Science & Technology, Japan
12:30 pm – 1:00 pm
SFRRI General Assembly
1:00 pm – 2:30 pm
Lunch (attendees on own)
2:30 pm – 4:30 pm
Oral presentations from submitted abstracts
(4 concurrent sessions)
4:30 pm – 6:30 pm
Formal Poster Presentations
7:30 pm – 9:30 pm
Closing/Awards Banquet
9:30 pm – 12:00 am
Hospitality
SUNDAY, NOVEMBER 21, 2010
PLENARY SESSION
Nitric Oxide and Oxygen: Co-Conspirators for Life
Chairs: Sruti Shiva, Ph.D., University of Pittsburgh and Douglas Thomas, Ph.D., University of Illinois at Chicago
Since its initial characterization as endothelial derived relaxing
factor, nitric oxide (NO) has been tightly linked to oxygen (O2).
The unique relationship between these two molecules is important for
most physiological and pathological conditions, ranging from cellular
bioenergetics to inflammation and adaptation to high altitude. This
session will focus on the different mechanisms by which NO and O2 modulate the production and metabolism of one another as well as their interactions with specific protein targets.
9:00 am – 9:30 am
NO Interactions with Mitochondrial Cytochrome c and Cytochrome Oxidase
Chris Cooper, Ph.D., University of Essex, UK
9:30 am – 10:00 am
Oxygen-Dependent Regulation of NO Production
Cynthia Otto, Ph.D., University of Pennsylvania
10:00 am – 10:30 am
Break
10:30 am – 11:00 am
Recent Insights into the Biological Signaling Properties of Sodium Nitrite
Mark Gladwin, MD, University of Pittsburgh
11:00 am – 11:30 am
Nitric Oxide Levels and Adaptation to High Altitude Hypoxia
Serpil Erzurum, MD, The Cleveland Clinic
11:30 am
Adjourn