New Approaches for Examining Nitrative and Oxidative Stress in Biology
Wednesday, November 17, 2010
8:30 am - 3:40 pm
Chairs: Joe S. Beckman, Ph.D., Linus Pauling Institute and Balaraman Kalyanaraman, Ph.D., Medical College of Wisconsin
Major experimental advances made in the past several years can fundamentally transform our ability to critically examine the biological role of nitrative and oxidative stress in biology and medicine. New fluorescent probes can detect superoxide and peroxynitrite production in vivo, but only if carefully applied. Nitration, hydroxylation and dimerization of protein-bound tyrosines have become a widely used marker of oxidative stress. New approaches for understanding the specificity for certain tyrosine sites to be susceptible to nitration will allow the biological implications of tyrosine modifications to be characterized. Importantly, the specific functional consequences can be determined by directly incorporating nitrotyrosine into recombinant proteins by unnatural amino acid mutagenesis. Mitochondria are key targets of oxidative stress. New technologies of monitoring mitochondrial function are transforming our understanding of their dysfunction in vivo. These new approaches can provide mechanistic insights into how oxidants specifically damage cells to produce pathological changes that underlie so many disease processes.
Registration fees for the workshop, which includes lunch and course materials, are not included in the annual meeting tuition. Persons wishing to attend the workshop must clearly indicate their participation on the registration form. Attendance will be limited to the first 175 applicants, so it is advisable to register early! Participants should plan to arrive in Orlando on Tuesday to be ready for the Wednesday morning start.
Cost is $175 per person for SFRBM & SFRRI members and $200 for non-members.